Tham khảo tài liệu 'báo cáo hóa học: " the internal initiation of translation in bovine viral diarrhea virus rna depends on the presence of an rna pseudoknot upstream of the initiation codon"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | BioMed Central Virology Journal Research Open Access The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon Lorin Moes1 2 and Manfred Wirth 2 Address 1Evolva CH-4123 Allschwil Switzerland and 2Molecular Biotechnology Helmholtz Centre for Infection Research HZI D-38124 Braunschweig Germany Email Lorin Moes - lorinm@ ManfredWirth - mwi@ Corresponding author Published 22 November 2007 Received 23 October 2007 Accepted 22 November 2007 Virology Journal 2007 4 124 doi 1743-422X-4-124 This article is available from http content 4 1 124 2007 Moes and Wirth licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Bovine viral diarrhea virus BVDV is the prototype representative of the pestivirus genus in the Flaviviridae family. It has been shown that the initiation of translation of BVDV RNA occurs by an internal ribosome entry mechanism mediated by the 5 untranslated region of the viral RNA 1 . The 5 and 3 boundaries of the IRES of the cytopathic BVDV NADL have been mapped and it has been suggested that the IRES extends into the coding of the BVDV polyprotein 2 . A putative pseudoknot structure has been recognized in the BVDV 5 UTR in close proximity to the AUG start codon. A pseudoknot structure is characteristic for flavivirus IRESes and in the case of the closely related classical swine fever virus CSFV and the more distantly related Hepatitis C virus HCV pseudoknot function in translation has been demonstrated. Results To characterize the BVDV IRESes in detail we studied the BVDV translational initiation by transfection of dicistronic expression plasmids into mammalian .
