Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans | Virology Journal BioMed Central Open Access Short report Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans Stella SF Ng Olive TW Li Timothy KW Cheung J S Malik Peiris and Leo LM PoOn Address State Key Laboratory of Emerging Infectious Diseases Department of Microbiology The University of Hong Kong Hong Kong SAR China Email Stella SF Ng - Stellang0713@ Olive TW Li - oliveli@ Timothy KW Cheung - kaiwing@ J S Malik Peiris - malik@ Leo LM Poon - llmpoon@ Corresponding author Published II January 2008 Received 10 October 2007 Accepted 1 1 January 2008 Virology Journal 2008 5 2 doi 1743-422X-5-2 This article is available from http content 5 1 2 2008 Ng et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ The initiation of transcription and replication of influenza A virus requires the 5 and 3 ends of vRNA. Here the role of segment-specific non-coding sequences of influenza A virus on viral RNA synthesis was studied. Recombinant viruses with the nonstructural protein NS segment-specific non-coding sequences replaced by the corresponding sequences of the neuraminidase NA segment were characterized. The NS and NA vRNA levels in cells infected with these mutants were much higher than those of the wild type whereas the NS and NA mRNA levels of the mutants were comparable to the wild-type levels. By contrast the PB2 vRNA and mRNA levels of all the tested viruses were similar indicating that vRNA with heterologous segment-specific non-coding sequences was not affected by the mutations. The observations
