Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein | Virology Journal BioMed Central Research Open Access p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein Craig Brown1 Anna M Kowalczyk1 Ewan R Taylor2 Iain M Morgan2 and Kevin Gaston 1 Address department of Biochemistry School of Medical School University of Bristol Bristol UK and institute of Comparative Medicine University of Glasgow Glasgow UK Email Craig Brown - Anna M Kowalczyk - Ewan RTaylor - Iain M Morgan - Kevin Gaston - Corresponding author Published II January 2008 Received 3 December 2007 Accepted 1 1 January 2008 Virology Journal 2008 5 5 doi 1743-422X-5-5 This article is available from http content 5 1 5 2008 Brown et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human papillomavirus HPV DNA replication can be inhibited by the cellular tumour suppressor protein p53. However the mechanism through which p53 inhibits viral replication and the role that this might play in the HPV life cycle are not known. The papillomavirus E2 protein is required for efficient HPV DNA replication and also regulates viral gene expression. E2 represses transcription of the HPV E6 and E7 oncogenes and can thereby modulate indirectly host cell proliferation and survival. In addition the E2 protein from HPV 16 has been shown to bind p53 and to be capable of inducing apoptosis independently of E6 and E7. Results Here we use a panel of E2 mutants to confirm that mutations which block the induction of apoptosis via this E6 E7-independent pathway have little or no effect on the induction of apoptosis by the E6 E7-dependent pathway. .
