Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells | Virology Journal BioMed Central Research Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells Juan E Ludert Clemente Mosso Ivonne Ceballos-Olvera and Rosa M del Angel Open Access Address Departamento de Patología Experimental Centro de Investigation y de Estudios Avanzados del . Mexico City Mexico Email Juan E Ludert - jludert@ Clemente Mosso - clemen_elche@ Ivonne Ceballos- Olvera - ivonneceol@ Rosa M del Angel - rmangel@ Corresponding author Published 25 April 2008 Received 12 February 2008 Accepted 25 April 2008 Virologyjournal 2008 5 51 doi 1743-422X-5-51 This article is available from http content 5 1 51 2008 Ludert et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Current methods for dengue virus quantitation are either time consuming technically demanding or costly. As an alternative the commercial enzyme immunoassay Platelia Dengue NS1 AG BioRad Laboratories was used to monitor semiquantitatively dengue virus replication in cultured cells. The presence of NS1 protein was evaluated in supernatants from Vero and C6 36 HT cells infected with dengue virus. The amount of NS1 detected in the supernatants of infected cells was proportional to the initial MOI used and to the time of post infection harvest. This immunoassay was also able to detect the presence of NS1 in the supernatants of infected human macrophages. Inhibition of dengue virus replication in C6 36 HT cells treated with lysosomotropic drugs was readily monitored with the use of this assay. These results suggest that the Platelia Dengue NS1 AG kit can be used as a fast and reliable surrogate method for the relative quantitation of
