Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Expression of frog virus 3 genes is impaired in mammalian cell lines | Virology Journal BioMed Central Open Access Short report Expression of frog virus 3 genes is impaired in mammalian cell lines Heather E Eaton1 Julie Metcalf2 and Craig R Brunetti 1 Address Department of Biology Trent University Peterborough ON Canada and - Department of Laboratory Medicine and Pathobiology University of Toronto Toronto ON Canada Email Heather E Eaton - heathereaton@ Julie Metcalf - juliem@ Craig R Brunetti - craigbrunetti@ Corresponding author Published 21 July 2008 Received 28 May 2008 Virology Journal 2008 5 83 doi 186 1743-422X-5-83 Accepted 21 July 2008 This article is available from http content 5 1 83 2008 Eaton et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Frog virus 3 FV3 is a large DNA virus that is the prototypic member of the family Iridoviridae. To examine levels of FV3 gene expression we generated a polyclonal antibody against the FV3 protein 75L. Following a FV3 infection in fathead minnow FHM cells 75L was found in vesicles throughout the cytoplasm as early as 3 hours post-infection. While 75L expressed strongly in FHM cells our findings revealed no 75L expression in mammalian cells lines despite evidence of a FV3 infection. One explanation for the lack of gene expression in mammalian cell lines may be inefficient codon usage. As a result 75L was codon optimized and transfection of the codon optimized construct resulted in detectable expression in mammalian cells. Therefore although FV3 can infect and replicate in mammalian cell lines the virus may not express its full complement of genes due to inefficient codon usage in mammalian species. Background Iridoviridae family members are large icosahedral double-stranded DNA .
