Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Discovery of frameshifting in Alphavirus 6K resolves a 20-year enigma | Virology Journal o BioMed Central Discovery of frameshifting in Alphavirus 6K resolves a 20-year enigma Andrew E Firth 11 Betty YW Chung11 Marina N Fleeton12 and John F Atkins 1 3 Address 1BioSciences Institute University College Cork Cork Ireland 2Department of Microbiology Moyne Institute for Preventive Medicine Trinity College Dublin 2 Ireland and 3Department of Human Genetics University of Utah Salt Lake City UT 84112-5330 USA Email Andrew E Firth - Betty YW Chung - Marina N Fleeton - fleetonm@ John F Atkins - Corresponding authors tEqual contributors Published 26 September 2008 Received 27 August 2008 Virology Journal 2008 5 108 doi 1743-422X-5-108 Accepted 26 September 2008 This article is available from http content 5 1 108 2008 Firth et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The genus Alphavirus includes several potentially lethal human viruses. Additionally species such as Sindbis virus and Semliki Forest virus are important vectors for gene therapy vaccination and cancer research and important models for virion assembly and structural analyses. The genome encodes nine known proteins including the small 6K protein. 6K appears to be involved in envelope protein processing membrane permeabilization virion assembly and virus budding. In protein gels 6K migrates as a doublet - a result that to date has been attributed to differing degrees of acylation. Nonetheless despite many years of research its role is still relatively poorly understood. Results We report that ribosomal - I frameshifting with an estimated efficiency of I0-I8 occurs at a conserved UUUUUUA motif within the sequence encoding 6K .
