Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Plaque assay for human coronavirus NL63 using human colon carcinoma cells | Virology Journal BioMed Central Open Access Methodology Plaque assay for human coronavirus NL63 using human colon carcinoma cells Petra Herzog1 3 Christian Drosten 2 and Marcel A Muller2 Address 1Bernhard Nocht Institute for Tropical Medicine Bernhard-Nocht-Str. 74 D-20359 Hamburg Germany institute of Virology University of Bonn Medical Centre Sigmund-Freud-Str. 25 53127 Bonn Germany and 3Qiagen Hamburg GmbH Kõnigstr. 4a D-22767 Hamburg Germany Email Petra Herzog - herzog@ Christian Drosten - drosten@ Marcel A Muller - muller@ Corresponding author Published 12 November 2008 Received 22 October 2008 Accepted 12 November 2008 Virology Journal 2008 5 138 doi l743-422X-5-l38 This article is available from http content 5 l l38 2008 Herzog et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Coronaviruses cause a broad range of diseases in animals and humans. Human coronavirus hCoV NL63 is associated with up to 10 of common colds. Viral plaque assays enable the characterization of virus infectivity and allow for purifying virus stock solutions. They are essential for drug screening. Hitherto used cell cultures for hCoV-NL63 show low levels of virus replication and weak and diffuse cytopathogenic effects. It has not yet been possible to establish practicable plaque assays for this important human pathogen. Results 12 different cell cultures were tested for susceptibility to hCoV-NL63 infection. Human colon carcinoma cells CaCo-2 replicated virus more than 100 fold more efficiently than commonly used African green monkey kidney cells LLC-MK2 . CaCo-2 cells showed cytopathogenic effects 4 days post infection. Avicel agarose and .
