Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Evaluation of three Polymerase chain reaction tests targeting morphological transforming region II, UL-83 gene and glycoprotein O gene for the detection of Human Cytomegalovirus genome in clinical specimens of immunocompromised patients in Chennai, India | Virology Journal BioMed Central Research Open Access Evaluation of three Polymerase chain reaction tests targeting morphological transforming region II UL-83 gene and glycoprotein O gene for the detection of Human Cytomegalovirus genome in clinical specimens of immunocompromised patients in Chennai India P Sowmya HN Madhavan and KLTherese Address L T Microbiology Research Center Vision Research Foundation 18 College Road Chennai - 600-006 India Email P Sowmya - p_sowmya_79@ HN Madhavan - drhnm@ KLTherese - lilyirudayam@ Corresponding author Published 30 March 2006 Received 10 November 2005 Accepted 30 March 2006 Virology Journal2006 3 20 doi 1743-422X-3-20 This article is available from http content 3 1 20 2006Sowmya et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human Cytomegalovirus HCMV continues to be an important cause of morbidity and occasional mortality in immunocompromised patients. Polymerase chain reaction PCR is the most sensitive and commonly used method for the assessment of HCMV infection in the immunocompromised patients at risk from severe associated clinical manifestations. However there is little consistency in the qualitative PCR used for different regions of HCMV genome. Therefore the performance of three Qualitative PCR tests to detect HCMV genome in clinical specimens from immunocompromised patients was evaluated. With pp65 antigenemia assay as the gold standard nested PCR for morphological transforming region II mtr II and glycoprotein O gO gene and uniplex PCR for UL 83 gene were applied on 92 consecutive clinical specimens obtained from 74 immunocompromised patients with clinically suspected HCMV disease. Virus .
