Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology | Virology Journal BioMed Central Research Open Access Effect of Ebola virus proteins GP NP and VP35 on VP40 VLP morphology Reed F Johnson1 Peter Bell2 and Ronald N Harty 1 Address Department of Pathobiology School of Veterinary Medicine University of Pennsylvania 3800 Spruce St. Philadelphia PA 19104 USA and 2Gene Therapy Program Department of Pathology and Laboratory Medicine School of Medicine University of Pennsylvania Philadelphia PA 19104 USA Email Reed F Johnson - rfj@ Peter Bell - pbell@ Ronald N Harty - rharty@ Corresponding author Published 23 May 2006 Received 26 April 2006 Accepted 23 May 2006 Virology Journal 2006 3 31 doi 186 1743-422X-3-31 This article is available from http content 3 1 31 2006 Johnson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Recently we described a role for Ebola virus proteins NP GP and VP35 in enhancement of VP40 VLP budding. To explore the possibility that VLP structure was altered by co-expression of EBOV proteins leading to the observed enhancement of VP40 VLP budding we performed density gradient analysis as well as electron microscopy studies. Our data suggest that VP40 is the major determinant of VLP morphology as co-expression of NP GP and VP35 did not significantly change VLP density length and diameter. Ultra-structural changes were noted in the core of the VLPs when NP was co-expressed with VP40. Overall these findings indicate that major changes in morphology of VP40 VLPs were likely not responsible for enhanced budding of VP40 VLPs in the presence of GP NP and or VP35. Introduction Ebola and Marburg viruses are members of the Filoviridae family of the order Mononegavirales. Both viruses are .
