Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The temperature arrested intermediate of virus-cell fusion is a functional step in HIV infection | Virology Journal BioMed Central Research Open Access The temperature arrested intermediate of virus-cell fusion is a functional step in HIV infection Hamani I Henderson1 and Thomas J Hope 2 Address University of Illinois @ Chicago Department of Microbiology and Immunology Chicago IL 60612 USA and 2Northwestern University Department of Cell and Molecular Biology Chicago IL 60611 USA Email Hamani I Henderson - hamani@ Thomas J Hope - thope@ Corresponding author Published 25 May 2006 Received 15 April 2006 Accepted 25 May 2006 Virology Journal 2006 3 36 doi 186 1743-422X-3-36 This article is available from http content 3 1 36 2006 Henderson and Hope licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV entry occurs via membrane-mediated fusion of virus and target cells. Interactions between gp120 and cellular co-receptors lead to both the formation of fusion pores and release of the HIV genome into target cells. Studies using cell-cell fusion assays have demonstrated that a temperature-arrested state TAS can generate a stable intermediate in fusion related events. Other studies with MLV pseudotyped with HIV envelope also found that a temperature sensitive intermediate could be generated as revealed by the loss of a fluorescently labeled membrane. However such an intermediate has never been analyzed in the context of virus infection. Therefore we used virus-cell infection with replication competent HIV to gain insights into viruscell fusion. We find that the TAS is an intermediate in the process culminating in the HIV infection of a target cell. In the virion-cell TAS CD4 has been engaged the heptad repeats of gp41 are exposed and the complex is kinetically .
