Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production | Virology Journal BioMed Central Research Caveolin-I interacts with the Gag precursor of murine leukaemia virus and modulates virus production Zheng Yu 1 Christiane Beer 1 2 Mario Koester1 and Manfred Wirth 1 Open Access Address Molecular Biotechnology Division German Research Centre for Biotechnology GBF Mascheroder Weg 1 Braunschweig Germany and 2Department of Molecular Biology Aarhus University . Mollers Alle 130 Aarhus Denmark Email Zheng Yu - zyu@ Christiane Beer-chb@ Mario Koester-mks@ Manfred Wirth - mwi@ Corresponding author fEqual contributors Published 06 September 2006 Received 02 June 2006 Accepted 06 September 2006 Virology journal 2006 3 73 doi 1743-422X-3-73 This article is available from http content 3 1 73 2006 Yu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Retroviral Gag determines virus assembly at the plasma membrane and the formation of virus-like particles in intracellular multivesicular bodies. Thereby retroviruses exploit by interaction with cellular partners the cellular machineries for vesicular transport in various ways. Results The retroviral Gag precursor protein drives assembly of murine leukaemia viruses MLV at the plasma membrane PM and the formation of virus like particles in multivesicular bodies MVBs . In our study we show that caveolin-l Cav-I a multifunctional membrane-associated protein co-localizes with Gag in a punctate pattern at the PM of infected NIH 3T3 cells. We provide evidence that Cav-1 interacts with the matrix protein MA of the Gag precursor. This interaction is mediated by a Cav-1 binding domain CBD within the .
