Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Infection of human cytomegalovirus in cultured human gingival tissue | Virology Journal BioMed Central Research Open Access Infection of human cytomegalovirus in cultured human gingival tissue Rong Hai Alice Chu Hongjian Li Sean Umamoto Paul Rider and Fenyong Liu Address Program in Infectious Diseases and Immunity Program in Comparative Biochemistry School of Public Health 140 Warren Hall University of California Berkeley CA 94720 USA Email Rong Hai - ronghai@ Alice Chu - snickers654@ Hongjian Li - hongjian6502@ Sean Umamoto - uma888@ Paul Rider - pjrider@ Fenyong Liu - liu_fy@ Corresponding author Published 05 October 2006 Received 07 July 2006 Accepted 05 October 2006 Virology Journal 2006 3 84 doi 1743-422X-3-84 This article is available from http content 3 1 84 2006 Hai et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human cytomegalovirus HCMV infection in the oral cavity plays an important role in its horizontal transmission and in causing viral-associated oral diseases such as gingivitis. However little is currently known about HCMV pathogenesis in oral mucosa partially because HCMV infection is primarily limited to human cells and few cultured tissue or animal models are available for studying HCMV infection. Results In this report we studied the infection of HCMV in a cultured gingival tissue model EpiGingival MatTek Co. and investigated whether the cultured tissue can be used to study HCMV infection in the oral mucosa. HCMV replicated in tissues that were infected through the apical surface achieving a titer of at least 300-fold at 10 days postinfection. Moreover the virus spread from the apical surface to the basal region and reduced the thickness of the stratum
