Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Vaccinia virus replication is not affected by APOBEC3 family members | Virology Journal BioMed Central Research Vaccinia virus replication is not affected by APOBEC3 family members Melanie Kremer Yasemin Suezer Yolanda Martinez-Fernandez Carsten Munk Gerd Sutter and Barbara S Schnierle Open Access Address Paul-Ehrlich-Institut Paul-Ehrlich-Str. 51-59 63225 Langen Germany Email Melanie Kremer - kreme@ Yasemin Suezer - sueya@ Yolanda Martinez-Fernandez - maryo@ Carsten Munk - mueca@ Gerd Sutter - sutge@ Barbara S Schnierle - schba@ Corresponding author Published 19 October 2006 Received 30 August 2006 Accepted 19 October 2006 Virology journal 2006 3 86 doi l743-422X-3-86 This article is available from http content 3 1 86 2006 Kremer et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background The APOBEC3G protein represents a novel innate defense mechanism against retroviral infection. It facilitates the deamination of the cytosine residues in the single stranded cDNA intermediate during early steps of retroviral infection. Most poxvirus genomes are relatively A T-rich which may indicate APOBEC3G-induced mutational pressure. In addition poxviruses replicate exclusively in the cytoplasm where APOBEC3G is located. It was therefore tempting to analyze whether vaccinia virus replication is affected by APOBEC3G. Results The replication of vaccinia virus a prototype poxvirus was not however inhibited in APOBEC3G-expressing cells nor did other members of the APOBEC3 family alter vaccinia virus replication. HIV counteracts APOBEC3G by inducing its degradation. However Western blot analysis showed that the levels of APOBEC3G protein were not affected by vaccinia .
