Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells | Matsushima et al. Journal of Translational Medicine 2011 9 30 http content 9 1 30 TRANSLATIONAL MEDICINE RESEARCH Open Access MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells 1 f f 1 1 Kayoko Matsushima Hajime Isomoto Naoyuki Yamaguchi Naoki Inoue Haruhisa Machida 3 3 I Ỉ4 lz- 4 I4 Toshiyuki Nakayama Tomayoshi Hayashi Masaki Kunizaki Shigekazu Hidaka Takeshi Nagayasu l l coHIrr t M lz chim 5 I líiíũ ilzi 16 Metric K lifci li lzo6 Al iro OHfci in I6 Chi inir Hi Vom ck iii 36 MantN 17 Masahiro Nakashima Kenta Ujifuku Norisato Mitsutake Akira Ohtsuru Shunichi Yamashita Manav Korpal Yibin Kang7 Philip A Gregory8 Gregory J Goodall8 Shigeru Kohno2 and Kazuhiko Nakao1 Abstract Background Esophageal squamous cell carcinoma ESCC is often diagnosed at later stages until they are incurable. MicroRNA miR is a small non-coding RNA that negatively regulates gene expression mainly via translational repression. Accumulating evidence indicates that deregulation of miR is associated with human malignancies including ESCC. The aim of this study was to identify miR that could be specifically expressed and exert distinct biological actions in ESCC. Methods Total RNA was extracted from ESCC cell lines OE21 and TE10 and a non-malignant human esophageal squamous cell line Het-1A and subjected to microarray analysis. Expression levels of miR that showed significant differences between the 2 ESCC and Het-1A cells based on the comprehensive analysis were analyzed by the quantitative reverse transcriptase RT -PCR method. Then functional analyses including cellular proliferation apoptosis and Matrigel invasion and the wound healing assay for the specific miR were conducted. Using ESCC tumor samples and paired surrounding non-cancerous tissue obtained endoscopically the association with histopathological differentiation was examined with .
