Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Periostin: a promising target of therapeutical intervention for prostate cancer | Sun et al. Journal of Translational Medicine 2011 9 99 http content 9 1 99 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Periostin a promising target of therapeutical intervention for prostate cancer 1 z 71 2 1 1 3 1 1 z z 1 Chuanyu Sun Xiaojun Zhao Ke Xu Jian Gong Weiwei Liu Weihong Ding Yuancheng Gou Guowei Xia and Qiang Ding1 Abstract Background In our recent study Periostin was up-regulated in prostate cancer PCa compared with benign prostate hyperplasia BPH by proteomics analysis of prostate biopsies. We investigated the effect of sliencing Periostin by RNA interference RNAi on the proliferation and migration of PCa LNCap cell line. Methods All the prostate biopsies from PCa BPH and BPH with local prostatic intraepithelial neoplasm PIN were analyzed by iTRAQ Isobaric tags for relative and absolute quantification technology. Western blotting and immunohistochemical staining were used to verify Periostin expression in the tissues of PCa. Periostin expression in different PCa cell lines was determined by immunofluorescence staining western blotting and reverse transcription PCR RT-PCR . The LNCap cells with Periostin expression were used for transfecting shRNA-Periostin lentiviral particles. The efficancy of transfecting shRNA lentiviral particles was evaluated by immunofluorescence western blotting and Real-time PCR. The effect of silencing Periostin expression by RNAi on proliferation of LNCap cells was determined by MTT assay and tumor xenografts. The tissue slices from theses xenografts were analyzed by hematoxylin and eosin HE staining. The expression of Periostin in the xenografts was deteminned by Immunohistochemical staining and western blotting. The migration of LNCap cells after silencing Periostin gene expression were analyzed in vitro. Results Periostin as the protein of interest was shown fold up-regulation in PCa compared with BPH. The overexpression of Periostin in the stroma of PCa was confirmed by .
