Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons | Virology Journal BioMed Central Methodology Open Access Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons A Gregory Bruce Angela M Bakke Margaret E Thouless and Timothy M Rose Address Department of Pathobiology School of Public Health and Community Medicine University of Washington Seattle WA 98195 USA Email A Gregory Bruce - bruceg@ Angela M Bakke - bakkea@ Margaret E Thouless - methoul@ Timothy M Rose - trose@ Corresponding author Published 05 January 2005 Received 16 November 2004 Accepted 05 January 2005 Virology Journal 2005 2 2 doi l743-422X-2-2 This article is available from http content 2 1 2 2005 Bruce et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Two distinct lineages of rhadinoviruses related to Kaposi s sarcoma-associated herpesvirus KSHV HHV8 have been identified in macaques and other Old World non-human primates. We have developed a real-time quantitative PCR QPCR assay using a TaqMan probe to differentially detect and quantitate members of the rhadinovirus-2 RV2 lineage. PCR primers were derived from sequences within ORF 60 and the adjacent ORF 59 60 intergenic region which were highly conserved between the macaque RV2 rhadinoviruses rhesus rhadinovirus RRV and Macaca nemestrina rhadinovirus-2 MneRV2 . These primers showed little similarity to the corresponding sequences of the macaque RV1 rhadinoviruses retroperitoneal fibromatosis herpesvirus Macaca nemestrina RFHVMn and Macaca mulatta RFHVMm . To determine viral loads per cell an additional TaqMan QPCR assay was developed to detect the single copy cellular oncostatin M .
