Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Physicochemical characterization of vibriophage N5 | Virology Journal BioMed Central Research Physicochemical characterization of vibriophage N5 Anindito Sen1 2 and Amar N Ghosh 1 Open Access Address 1Division of Electron Microscopy National Institute of Cholera and Enteric Diseaess P-33 . Road Scheme- XM Beleghata Kolkata-700010. India and 2 Present Address Laboratory of Structural Biology Room 1504 Building 50 NIAMS NIH Bethesda MD 20852 USA Email Anindito Sen - sena@ Amar N Ghosh - ghoshan@ Corresponding author Published II April 2005 Received 20 December 2004 Accepted 1 1 April 2005 Virology Journal 2005 2 27 doi 1 743-422X-2-27 This article is available from http content 2 1 27 2005 Sen and Ghosh licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Phage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor Ghosh A. N. Ansari M. Q. and Dutta G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70 2241-2243 1989 . In the present communication the growth curve molecular weight and confirmation of the genome partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has nonpermuted invariant sequence. Presence of cohesive ends has also been documented. Vibrio cholerae the causative agent of cholera in humansis classified into two serotypes O1 and nonO1 1 . The O1 strains are divided into two biotypes Classical and El Tor. Before 1961 most epidemics had been caused by the classical biotype. However after 1961 the El Tor strains became the main causative agent of the cholera. Phage typing has proved to be useful and successful tool to tract down the spread of this dreadful disease.
