Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Matrix attachment regions as targets for retroviral integration | Virology Journal BioMed Central Research Open Access Matrix attachment regions as targets for retroviral integration Chassidy N Johnson and Laura S Levy Address Department of Microbiology Immunology and Tulane Cancer Center Tulane University School of Medicine New Orleans Louisiana 70112 USA Email ChassidyN Johnson - cjohnso9@ LauraS Levy - llevy@ Corresponding author Published 19 August 2005 Received 13 June 2005 Accepted 19 August 2005 Virology Journal 2005 2 68 doi 1743-422X-2-68 This article is available from http content 2 1 68 2005 Johnson and Levy licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The randomness of retroviral integration has been debated for many years. Recent evidence indicates that integration site selection is not random and that it is influenced by both viral and cellular factors. To study the role of DNA structure in site selection retroviral integration near matrix attachment regions MARs was analyzed for three different groups of retroviruses. The objective was to assess whether integration near MARs may be a factor for integration site selection. Results Results indicated that MLV SL3-3 MuLV HIV-1 and HTLV-1 integrate preferentially near MARs specifically within 2-kilobases kb . In addition a preferential position and orientation relative to the adjacent MAR was observed for each virus. Further analysis of SL3-3 MuLV insertions in common integration sites CISs demonstrated a higher frequency of integration near MARs and an orientation preference that was not observed for integrations outside CISs. Conclusion These findings contribute to a growing body of evidence indicating that retroviral integration is not random that MARs .
