Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Expression of interleukin-1 (IL-1) ligands system in the most common endometriosis-associated ovarian cancer subtypes | Keita et al. Journal of Ovarian Research 2010 3 3 http content 3 1 3 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Expression of interleukin-1 IL-1 ligands system in the most common endometriosis-associated ovarian cancer subtypes Mamadou Keita1 Paul Bessette1 Manuella Pelmus2 Youssef Ainmelk1 Aziz Aris1 3 Abstract Objectives Endometrioid carcinoma of the ovary is one of the most types of epithelial ovarian cancer associated to endometrioisis. Endometrioid tumors as well as endometriotic implants are characterized by the presence of epithelial cells stromal cells or a combination of booth that resemble the endometrial cells suggesting a possible endometrial origin of these tumors. Pro-inflammatory cytokines including interleukin-1 IL-1 have been reported to be involved in both endometriosis and ovarian carcinogenesis. The major objective of this study was to determine the level expression of IL-1 ligands system IL-1a IL-1Ị3 and IL-1RA in the most common subtypes of ovarian cancer cells compared to endometrial cells. Methods We used primary endometrial cells endometrial cell line RL-952 and different subtypes of epithelial ovarian cancer cell lines including TOV-112D endometrioid TOV-21G clear cell and OV-90 serous . Immunofluorescence and real-time PCR analysis were used respectively for detecting IL-1 ligands at the levels of cell-associated protein and mRNA. Soluble IL-1 ligands were analyzed by ELISA. Results We demonstrated that IL-1 ligands were expressed by all endometriosis-associated ovarian cancer subtypes and endometrial cells. In contrast to other cancer ovarian cells endometrioid cells exhibit a specific decrease of cell-associated IL-1RA expression and its soluble secretion. Conclusion Endometrioid ovarian cancer exhibits an alteration in the expression of IL-1RA a key protector against tumorogenic effects of IL-1. This alteration evokes the same alteration observed in endometriotic cells in previous studies. This suggests a
