Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth | Yallapu et al. Journal of Ovarian Research 2010 3 11 http content 3 1 11 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Curcumin induces chemo radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth Murali M Yallapu11 Diane M Mahert1 Vasudha Sundram 1 Maria C Bell2 Meena Jaggi1 2 and Subhash C Chauhan 1 2 Abstract Background Chemo radio-resistance is a major obstacle in treating advanced ovarian cancer. The efficacy of current treatments may be improved by increasing the sensitivity of cancer cells to chemo radiation therapies. Curcumin is a naturally occurring compound with anti-cancer activity in multiple cancers however its chemo radio-sensitizing potential is not well studied in ovarian cancer. Herein we demonstrate the effectiveness of a curcumin pre-treatment strategy for chemo radio-sensitizing cisplatin resistant ovarian cancer cells. To improve the efficacy and specificity of curcumin induced chemo radio sensitization we developed a curcumin nanoparticle formulation conjugated with a monoclonal antibody specific for cancer cells. Methods Cisplatin resistant A2780CP ovarian cancer cells were pre-treated with curcumin followed by exposure to cisplatin or radiation and the effect on cell growth was determined by MTS and colony formation assays. The effect of curcumin pre-treatment on the expression of apoptosis related proteins and p-catenin was determined by Western blotting or Flow Cytometry. A luciferase reporter assay was used to determine the effect of curcumin on p-catenin transcription activity. The poly lactic acid-co-glycolic acid PLGA nanoparticle formulation of curcumin Nano-CUR was developed by a modified nano-precipitation method and physico-chemical characterization was performed by transmission electron microscopy and dynamic light scattering methods. Results Curcumin pre-treatment considerably reduced the dose of cisplatin and radiation required to inhibit the growth .
