Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor | Komarova et al. Journal of Ovarian Research 2010 3 12 http content 3 1 12 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor Svetlana Komarova 1 2 3 4 Justin Roth1 2 3 4 Ronald Alvarez5 David T Curiel1 2 3 4 and Larisa Pereboeva 1 2 3 4 Abstract Background Mesenchymal Progenitor Stem Cells MSC respond to homing cues providing an important mechanism to deliver therapeutics to sites of injury and tumors. This property has been confirmed by many investigators however the efficiency of tumor homing needs to be improved for effective therapeutic delivery. We investigated the feasibility of enhancing MSC tumor targeting by expressing an artificial tumor-binding receptor on the MSC surface. Methods Human MSC expressing an artificial receptor that binds to erbB2 a tumor cell marker were obtained by transduction with genetically modified adenoviral vectors encoding an artificial receptor MSC-AR . MSC-AR properties were tested in vitro in cell binding assays and in vivo using two model systems transient transgenic mice that express human erbB2 in the lungs and ovarian xenograft tumor model. The levels of luciferase-labeled MSCs in erbB2-expressing targeted sites were evaluated by measuring luciferase activity using luciferase assay and imaging. Results The expression of AR enhanced binding of MSC-AR to erbB2-expressing cells in vitrO compared to unmodified MSCs. Furthermore we have tested the properties of erbB2-targeted MSCs in vivo and demonstrated an increased retention of MSC-AR in lungs expressing erbB2. We have also confirmed increased numbers of erbB2-targeted MSCs in ovarian tumors compared to unmodified MSC. The kinetic of tumor targeting by ip injected MSC was also investigated. Conclusion These data demonstrate that targeting abilities of MSCs can be enhanced via introduction of artificial receptors. The application of this strategy for tumor cell-based delivery
