Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Neuronal transcription factor Brn-3a(l) is over expressed in high-grade ovarian carcinomas and tumor cells from ascites of patients with advanced-stage ovarian cancer | Ahmed et al. Journal of Ovarian Research 2010 3 17 http content 3 1 17 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Neuronal transcription factor Brn-3a l is over expressed in high-grade ovarian carcinomas and tumor cells from ascites of patients with advanced-stage ovarian cancer l l I I zh t A h mprl 1 2 3 4A rH n I irtifi1 3 r-l r ln R Rilnv1 nrt V RinHIrrv1 2 4 i lich in A Oi linn1 2 Nuziiat nllllleU Ardian Latili Clyde B Alley Jock K Findlay Miciiael A Quinn Abstract Objectives In view of the recent association of Brn-3 transcription factors with neuroblastomas cervical breast and prostate cancers we examined the expression of Brn-3a l in normal ovaries and in different histological grades of ovarian tumors. The expression of Brn-3a l was also evaluated in normal ovarian and cancer cell lines and tumor cells isolated from the ascites of advanced-stage ovarian cancer patients. Methods Normal ovaries benign borderline grades 1 2 and 3 ovarian tumors were analyzed by immunohistochemistry for Brn-3a l expression. A total of 46 ovarian specimens were included in the study. Immunofluorescence was used to investigate the expression of Brn-3a in normal ovarian and cancer cell lines. Brn-3a l expression was also evaluated by Western blot in tumor cells isolated from ascites of advanced-stage ovarian cancer patients and also in ovarian cancer cell lines. Results Nearly 12 of normal and benign ovarian tissues and 57 of borderline ovarian tumors were positive for epithelial Brn-3a l expression. Stromal staining was higher and it constituted 40 of normal non-cancerous ovaries compared to 50 and 86 in benign and borderline tumors. On the other hand 85-100 of grades 1 2 3 ovarian tumors demonstrated nuclear and cytoplasmic Brn-3a l staining in the epithelium. Stromal staining in grades1 2 and 3 tumors constituted 71-88 of total staining. Overall immunoreactive Brn-3a was present in all grades of ovarian tumors. The extent of epithelial and stromal
