Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells | Ponnusamy et al. Journal of Ovarian Research 2011 4 7 http content 4 1 7 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells 1 1 11 Moorthy P Ponnusamy Parthasarathy Seshacharyulu ArokiaPriyanka Vaz Parama Dey and Surinder K Batra Abstract Background Recent evidence has suggested that the capability of cancer to grow propagate and relapse after therapy is dependent on a small subset of the cell population within the tumor called cancer stem cells. Therefore this subpopulation of cells needs to be targeted with different approaches by identification of unique stem-cell specific target antigens. One of the well known tumor antigens is the epithelial cell mucin MUC4 which is aberrantly expressed in ovarian cancer as compared to the normal ovary and plays a pivotal role in the aggressiveness and metastasis of ovarian cancer cells. In the present study we aimed to analyze the cancer stem cell population in MUC4 overexpressed ovarian cancer cells. Methods MUC4 was ectopically overexpressed in SKOV3 ovarian cancer cells. Western blot analysis was performed for MUC4 HER2 CD133 ALDH1 and Shh expression in MUC4 overexpressed cells. Confocal analysis of MUC4 HER2 and CD133 was also done in the MUC4 overexpressed cells. CD133 and Hoechst33342 dye staining was used to analyze the cancer stem cell population via FACS method in SKOV3-MUC4 cells. Results MUC4 overexpressed SKOV3 cells showed an increased expression of HER2 compared to control cells. MUC4 overexpression leads to increased side population SP and CD133-positive cancer stem cells compared to the control cells. Interestingly the tumor sphere type circular colony formation was observed only in the MUC4 overexpressed ovarian cancer cells. Furthermore the cancer stem cell marker CD133 was expressed along with MUC4 in the isolated circular colonies as analyzed by both confocal and western blot
