Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs | Journal of the International AIDS Society BioMed Central Research article Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs Dan Turner1 Bluma Brenner2 Daniela Moisi3 Chen Liang4 and Mark A Wainberg 5 Open Access Address Fellow in HIV Medicine McGill University Montreal Quebec Canada 2Assistant Professor Department of Surgery McGill University Montreal Quebec Canada 3Research Associate McGill University Montreal Quebec Canada 4Assistant Professor Department of Microbiology McGill University Montreal Quebec Canada and 5Director McGill University AIDS Centre Montreal Quebec Canada Email Mark A Wainberg - Corresponding author Published 23 March 2005 Journal of the International AIDS Society 2005 7 69 This article is available from http content 7 1 69 Abstract The nucleotide transition G A is known as a hypermutation due to its high prevalence in HIV-1 and other pathogens. However the contribution of the G A transition in the generation of drug resistance mutations is unknown. Our objective was to ascertain the rate of nucleotide substitutions in protease PR and reverse transcriptase RT in both untreated and treated HIV-1 patients. Genotypic analysis was performed on viruses from both treated and untreated patients with subtype B infections. Nucleotide genomic diversity was compared with a consensus subtype B reference virus. Then the prevalence of resistance-associated mutations in different subgroups of treated patients was evaluated in relation to the patterns of nucleotide transitions. In untreated patients n 50 G A was most prevalent followed by A G C T and T C transitions. In treated patients n 51 the prevalence of A G was similar to that of G A. Among mutations that confer resistance to antiretroviral drugs M184V was present in 76 of treated patients and K70R in 31 A G transitions . Other frequent mutations in RT .
