Tham khảo tài liệu 'báo cáo hóa học: " functionalized halloysite nanotube-based carrier for intracellular delivery of antisense oligonucleotides"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Shi et al. Nanoscale Research Letters 2011 6 608 http content 6 1 608 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Functionalized halloysite nanotube-based carrier for intracellular delivery of antisense oligonucleotides Yin-Feng Shi Zhong Tian Yang Zhang He-Bai Shen and Neng-Qin Jia Abstract Halloysites are cheap abundantly available and natural with high mechanical strength and biocompatibility. In this paper a novel halloysite nanotube HNT -based gene delivery system was explored for loading and intracellular delivery of antisense oligodeoxynucleotides ASODNs in which functionalized HNTs f-HNTs were used as carriers and ASODNs as a therapeutic gene for targeting survivin. HNTs were firstly surface-modified with g-aminopropyltriethoxysilane in order to facilitate further biofunctionalization. The f-HNTs and the assembled f-HNT-ASODN complexes were characterized by transmission electron microscopy TEM dynamic light scattering UV-visible spectroscopy and fluorescence spectrophotometry. The intracellular uptake and delivery efficiency of the complexes were effectively investigated by TEM confocal microscopy and flow cytometry. In vitro cytotoxicity studies of the complexes using MTT assay exhibited a significant enhancement in the cytotoxic capability. The results exhibited that f-HNT complexes could efficiently improve intracellular delivery and enhance antitumor activity of ASODNs by the nanotube carrier and could be used as novel promising vectors for gene therapy applications which is attributed to their advantages over structures and features including a unique tubular structure large aspect ratio natural availability rich functionality good biocompatibility and high mechanical strength. Keywords halloysite nanotubes ASODNs cellular delivery cytotoxicity carrier. Introduction Gene therapy is attractive as a clinical treatment for cancers and genetic disorders. Antisense oligodeoxynucleotides ASODNs are .
