Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí hóa hoc quốc tế đề tài : Superhydrophobic poly(L-lactic acid) surface as potential bacterial colonization substrate | Sousa et al. AMB Express 2011 1 34 http content 1 1 34 o AMB Express a SpringerOpen Journal ORIGINAL Open Access Superhydrophobic poly L-lactic acid surface as potential bacterial colonization substrate Cláudia Sousa1 f Diana Rodrigues1 Rosário Oliveira1 Wenlong Song1 2 3 João F Mano1 2 3 and Joana Azeredo1 Abstract Hydrophobicity is a very important surface property and there is a growing interest in the production and characterization of superhydrophobic surfaces. Accordingly it was recently shown how to obtain a superhydrophobic surface using a simple and cost-effective method on a polymer named poly L-lactic acid PLLA . To evaluate the ability of such material as a substrate for bacterial colonization this work assessed the capability of different bacteria to colonize a biomimetic rough superhydrophobic SH PLLA surface and also a smooth hydrophobic H one. The interaction between these surfaces and bacteria with different morphologies and cell walls was studied using one strain of Staphylococcus aureus and one of Pseudomonas aeruginosa. Results showed that both bacterial strains colonized the surfaces tested although significantly higher numbers of S. aureus cells were found on SH surfaces comparing to H ones. Moreover scanning electron microscopy images showed an extracellular matrix produced by P. aeruginosa on SH PLLA surfaces indicating that this bacterium is able to form a biofilm on such substratum. Bacterial removal through lotus leaf effect was also tested being more efficient on H coupons than on SH PLLA ones. Overall the results showed that SH PLLA surfaces can be used as a substrate for bacterial colonization and thus have an exceptional potential for biotechnology applications. Keywords Poly L-lactic acid Superhydrophobicity Biomimetic surfaces Bacterial colonization substrate Introduction Industrial bioconversion processes can be performed using different kinds of reactors some of which are called immobilized cell reactors Tyagi
