Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population | Kohl et al. Nanoscale Research Letters 2011 6 505 http content 6 1 505 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population Vx cvmod kwhl1 rCo Cio I mr .cti no h2 A dIT Cioccollo3 AlHart ril ccFI2 dw . Ti RridCdo H I lorTcm T h i d I d bo4 Ivuiiiie Kohl Gertie J oostingh AUdn sossalla Albert Duschi nagen von Driesen and nagen Thielecke Abstract In most conventional in vitro toxicological assays the response of d complete cell population is averaged and therefore single-cell responses are not detectable. Such averaging might result in misinterpretations when only individual cells within a population respond to a certain stimulus. Therefore there is a need for non-invasive in vitro systems to verify the toxicity of nanoscale materials. In the present study a micro-sized cell culture chamber with a silicon nitride membrane mm2 was produced for cell cultivation and the detection of specific cell responses. The biocompatibility of the microcavity chip MCC was verified by studying adipogenic and neuronal differentiation. Thereafter the suitability of the MCC to study the effects of nanoparticles on a small cell population was determined by using a green fluorescence protein-based reporter cell line. Interleukin-8 promoter pIL8 induction a marker of an inflammatory response was used to monitor immune activation. The validation of the MCC-based method was performed using well-characterized gold and silver nanoparticles. The sensitivity of the new method was verified comparing the quantified pIL8 activation via MCC-based and standard techniques. The results proved the biocompatibility and the sensitivity of the microculture chamber as well as a high optical quality due to the properties of Si3N4. The MCC-based method is suited for threshold- and time-dependent analysis of .
