Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Cytotoxic effects and the mechanism of three types of magnetic nanoparticles on human hepatoma BEL-7402 cells | Kai et al. Nanoscale Research Letters 2011 6 480 http content 6 1 480 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Cytotoxic effects and the mechanism of three types of magnetic nanoparticles on human hepatoma BEL-7402 cells Wei Kai1 2 Xu Xiaojun3 Pu Ximing1 2 Hou Zhenqing1 2 and Zhang Qiqing1 2 4 Abstract The evaluation of the toxicity of magnetic nanoparticles MNPs has attracted much attention in recent years. The current study aimed to investigate the cytotoxic effects of Fe3O4 oleic acid-coated Fe3O4 OA-Fe3O4 and carbon-coated Fe C-Fe nanoparticles on human hepatoma BEL-7402 cells and the mechanisms. WST-1 assay demonstrated that the cytotoxicity of three types of MNPs was in a dose-dependent manner. G1 Fe3O4 and OA-Fe3O4 phase and G2 C-Fe phase cell arrests and apoptosis induced by MNPs were detected by flow cytometry analysis. The increase in apoptosis was accompanied with the Bax over-expression mitochondrial membrane potential decrease and the release of cytochrome C from mitochondria into cytosol. Moreover apoptosis was further confirmed by morphological and biochemical hallmarks such as swollen mitochondria with lysing cristae and caspase-3 activation. Our results revealed that certain concentrations of the three types of MNPs affect BEL-7402 cells viability via cell arrest and inducing apoptosis and the MNPs-induced apoptosis is mediated through the mitochondrial-dependent pathway. The influence potency of MNPs observed in all experiments would be C-Fe Fe3O4 OA-Fe3O4. Keywords magnetic nanoparticles BEL-7402 apoptosis mitochondrial-dependent pathway cell cycle Introduction Over the past few decades as nanotechnology and materials science has progressed incredibly swiftly nanomaterials have been widely applied in many fields including medicine pharmaceuticals manufacturing technologies electronics and telecommunications 1-3 . In particular the surge of interest in nanomaterials has significantly .
