Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy | Xing et al. Nanoscale Research Letters 2011 6 455 http content 6 1 455 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy Feiyue Xing1 Jiongkun Wang1 Mingqian Hu2 Yu Yu3 4 Guoliang Chen1 and Jing Liu5 Abstract A comparative study of immature and mature bone marrow-derived dendritic cells BMDCs was first performed through an atomic force microscope AFM to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger flatter and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs. Keywords dendritic cell nanostructure adhesion force comparison Introduction Dendritic cells DCs are the most potent specialized antigen-presenting cells which bridge the innate and adaptive immune response controlling both immunity and tolerance. It is well known that DCs may be derived from bone marrow progenitors with two major developmental stages immature and mature DCs 1 . The development of immature DCs can be induced with using cytokines such as granulocyte macrophage-colony stimulating factor GM-CSF 2 FMS-like tyrosine kinase 3 FLT3 3 or cytokine cocktails containing GM-CSF -IL-4 4 in vitro. After .
