Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Imaging the effect of receptor for advanced glycation endproducts on angiogenic response to hindlimb ischemia in diabetes | Tekabe et al. EJNMMI Research 2011 1 3 http content 1 1 3 9 EJNMMI Research a SpringerOpen Journal PRELIMINARY RESEARCH Open Access Imaging the effect of receptor for advanced glycation endproducts on angiogenic response to hindlimb ischemia in diabetes 1 1 4 5 Yared Tekabe Xiaoping Shen Joane Luma Drew Weisenberger Shi Fang Yan Roland Haubner Ann Marie Schmidt2 3 and Lynne Johnson1 Abstract Background Receptor for advanced glycation endproducts RAGE expression contributes to the impaired angiogenic response to limb ischemia in diabetes. The aim of this study was to detect the effect of increased expression of RAGE on the angiogenic response to limb ischemia in diabetes by targeting avp3 integrin with 99mTc-labeled Arg-Gly-Asp RGD . Methods Male wild-type WT C57BL 6 mice were either made diabetic or left as control for 2 months when they underwent femoral artery ligation. Four groups were studied at days 3 to 7 after ligation WT without diabetes NDM n 14 WT with diabetes DM n 14 RAGE- - NDM n 16 and RAGE- - DM n 14 . Mice were injected with 99mTc-HYNIC-RGD and imaged. Count ratios for ischemic non-ischemic limbs were measured. Muscle was stained for RAGE avp3 and lectins. Results There was no difference in count ratio between RAGE- - and WT NDM groups. Mean count ratio was lower for WT DM vs. WT NDM P . Mean count ratio was lower for the RAGE- - DM group than for RAGE- - NDM group vs. P and higher than for the WT DM group P . Immunohistopathology supported the scan findings. Conclusions In vivo imaging of avp3 integrin can detect the effect of RAGE on the angiogenic response to limb ischemia in diabetes. Background The prevalence of peripheral artery disease in the general population is 12 to 14 affecting 20 of those 70 years and contributes to significant morbidity. Limb ischemia in diabetics takes a particularly malignant course leading to impaired wound healing gangrene .
