Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of surface properties of silica nanoparticles on their cytotoxicity and cellular distribution in murine macrophages | Nabeshi et al. Nanoscale Research Letters 2011 6 93 http content 6 1 93 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Effect of surface properties of silica nanoparticles on their cytotoxicity and cellular distribution in murine macrophages Hiromi Nabeshi Tomoaki Yoshikawa Akihiro Arimori Tokuyuki Yoshida Saeko Tochigi Toshiro Hirai 12 2 2 2 3 24 1 Takanori Akase Kazuya Nagano Yasuhiro Abe Haruhiko Kamada Shin-ichi Tsunoda Norio Itoh Yasuo Yoshioka2 3 Yasuo Tsutsumi1 2 3 Abstract Surface properties are often hypothesized to be important factors in the development of safer forms of nanomaterials NMs . However the results obtained from studying the cellular responses to NMs are often contradictory. Hence the aim of this study was to investigate the relationship between the surface properties of silica nanoparticles and their cytotoxicity against a murine macrophage cell line . The surface of the silica nanoparticles was either unmodified nSP70 or modified with amine nSP70-N or carboxyl groups nSP70-C . First the properties of the silica nanoparticles were characterized. cells were then exposed to nSP70 nSP70-N or nSP70-C and any cytotoxic effects were monitored by analyzing DNA synthesis. The results of this study show that nSP70-N and nSP70-C have a smaller effect on DNA synthesis activity by comparison to unmodified nSP70. Analysis of the intracellular localization of the silica nanoparticles revealed that nSP70 had penetrated into the nucleus whereas nSP70-N and nSP70-C showed no nuclear localization. These results suggest that intracellular localization is a critical factor underlying the cytotoxicity of these silica nanoparticles. Thus the surface properties of silica nanoparticles play an important role in determining their safety. Our results suggest that optimization of the surface characteristics of silica nanoparticles will contribute to the development of .
