Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors | Nanoscale Res Lett 2010 5 1804-1811 DOI s11671-010-9716-9 NANO EXPRESS Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors Min Zhang Ya-Nan Xue Min Liu Ren-Xi Zhuo Shi-Wen Huang Received 11 April 2010 Accepted 26 July 2010 Published online 11 August 2010 The Author s 2010. This article is published with open access at Abstract An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N N -bis acryloyl cys-tamine with 90 yield. The polycationic micelles 198 nm mV prepared from the amphiphilic polyamidoamine by dialysis method can condense foreign plasmid DNA to form nanosized polycationic micelles DNA polyelectrolyte complexes with positive charges which transfected 293T cells with high efficiency. Under optimized conditions the transfection efficiencies of polycationic micelles DNA complexes are comparable to or even higher than that of commercially available branched PEI Mw 25 kDa . Keywords Gene delivery Polycationic micelles Biocleavable Introduction Polycationic gene delivery vectors have attracted more and more attention in the past decade because of their versatile chemical structures ease of preparation lack of immune response in vivo etc. 1-4 . Various cationic polymers have been applied in in vitro and in vivo gene delivery including polylysine PLL 5 6 polyethylenimine PEI 7 8 polyamidoamine dendrimer 9-12 cationic polyester 13 14 poly beta-aminoester 15 16 polyphosphoester and Min Zhang and Ya-Nan Xue contributed equally to this work. M. Zhang . Xue M. Liu . Zhuo . Huang Key Laboratory of Biomedical Polymers of Ministry of Education Department of Chemistry Wuhan University Wuhan 430072 People s Republic of China e-mail swhuang@ polyphosphoramidate 17-21 . In general water-soluble polycations were directly used to condense negatively charged DNA or RNA to from nanosized polyelectrolyte complexes polyplexes .
