Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Biocompatibility of Graphene Oxide | Wang et al. Nanoscale Res Lett 2011 6 8 http content 6 1 8 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Biocompatibility of Graphene Oxide Kan Wang1 Jing Ruanf Hua Song Jiali Zhang Yan Wo Shouwu Guo Daxiang Cui Abstract Herein we report the effects of graphene oxides on human fibroblast cells and mice with the aim of investigating graphene oxides biocompatibility. The graphene oxides were prepared by the modified Hummers method and characterized by high-resolution transmission electron microscope and atomic force microscopy. The human fibroblast cells were cultured with different doses of graphene oxides for day 1 to day 5. Thirty mice divided into three test groups low middle high dose and one control group were injected with and mg graphene oxides respectively and were raised for 1 day 7 days and 30 days respectively. Results showed that the water-soluble graphene oxides were successfully prepared graphene oxides with dose less than 20 gg mL did not exhibit toxicity to human fibroblast cells and the dose of more than 50 gg mL exhibits obvious cytotoxicity such as decreasing cell adhesion inducing cell apoptosis entering into lysosomes mitochondrion endoplasm and cell nucleus. Graphene oxides under low dose mg and middle dose mg did not exhibit obvious toxicity to mice and under high dose mg exhibited chronic toxicity such as 4 9 mice death and lung granuloma formation mainly located in lung liver spleen and kidney almost could not be cleaned by kidney. In conclusion graphene oxides exhibit dose-dependent toxicity to cells and animals such as inducing cell apoptosis and lung granuloma formation and cannot be cleaned by kidney. When graphene oxides are explored for in vivo applications in animal or human body its biocompatibility must be considered. Introduction In recent years a lot of engineered nanomaterials are fabricated endlessly and investigated for their applications 1-6 and
