Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots | Liang et al. Nanoscale Res Lett 2011 6 51 http content 6 1 51 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots Gaofeng Liang Chao Ma Yanliang Zhu Shuchun Li Youhua Shao Yong Wang Zhongdang Xiao Abstract Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction PCR . Here CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the performance of multi-PCR by reducing the formation of nonspecific products in the complex system but an excessive amount of CdTe QDs could suppress the PCR. The effects of QDs on PCR can be reversed by increasing the polymerase concentration or by adding bovine serum albumin BSA . The mechanisms underlying these effects were also discussed. The results indicated that CdTe QDs could be used to optimize the amplification products of the PCR especially in the multi-PCR system with different primers annealing temperatures which is of great significance for molecular diagnosis. Introduction The PCR has become one of the most popular tool for molecular biology and pathogen detection as a high sensitive technique 1-3 but it still requires careful optimization on reaction conditions in order to eliminate nonspecific products in most PCR experiments especially in multiplex PCR multi-PCR . Optimized parameters could include the type and concentration of the enzyme reaction buffer content time and temperature of the annealing extension process primer design and use of a rapid heating-cooling response PCR machine 4 5 . It was reported that a variety of organic chemicals including single-stranded DNA-binding proteins SSBs 6 amides and betaine 7 imidazole tetramethylammonium chloride TMAC and TMAC derivatives 6-9 could act as accelerants in PCR mixture to increase yield and specificity. As a .
