Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage | o AMB Express a SpringerOpen Journal Ratanapariyanuch et al. AMB Express 2012 2 5 http content 2 1 5 ORIGINAL Open Access Biorefinery process for protein extraction from oriental mustard Brassica juncea L. Czern. using ethanol stillage Kornsulee Ratanapariyanuch1 Robert T Tyler1 Youn Young Shim2 and Martin JT Reaney2 Abstract Large volumes of treated process water are required for protein extraction. Evaporation of this water contributes greatly to the energy consumed in enriching protein products. Thin stillage remaining from ethanol production is available in large volumes and may be suitable for extracting protein rich materials. In this work protein was extracted from ground defatted oriental mustard Brassica juncea L. Czern. meal using thin stillage. Protein extraction efficiency was studied at pHs between and and salt concentrations between X 10-2 and M. The optimum extraction efficiency was pH and M NaCl. Napin and cruciferin were the most prevalent proteins in the isolate. The isolate exhibited high in vitro digestibility and lysine content g 100 g of protein . No differences in the efficiency of extraction SDS-PAGE profile digestibility lysine availability or amino acid composition were observed between protein extracted with thin stillage and that extracted with NaCl solution. The use of thin stillage in lieu of water for protein extraction would decrease the energy requirements and waste disposal costs of the protein isolation and biofuel production processes. Keywords Biorefinery Protein extraction Thin stillage Mustard Salt concentration Ethanol Introduction Brassica spp. oilseeds are grown throughout the world as sources of vegetable oil and protein-rich animal feed Henriksen et al. 2009 . According to statistical data from the Canada Grains Council 2011 the average annual production of Canadian canola over the period 2001-2010 was million tonnes and the Canadian oilseed crushing .
