Tham khảo tài liệu 'new developments in biomedical engineering 2011 part 15', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 552 New Developments in Biomedical Engineering of tissue homogenates were collected and concentrated by centrifugation 14000 x g for 10 min. to obtain samples of ml volume. Electrophoretic studies were performed according to SDS NaCl Laemmli method 1970 . Electrophoresis was carried out in 10 separating gel with 4 stacking gel using voltage 140 V. Separated proteins were visualized in the gel using Coomassie Brillant Blue R 250 CBB dissolved in solution of methanol acetic acid water 25 10 65 . For destaining gels were incubated in the same solution without dye Cwalina et al. 2005 . The qualitative analyses of the electrophoregrams were performed using Biotec Fischer System. Histological studies were carried out under the Polyvar 2 - Leica light microscope under magnification 200X. Tissue samples were dehydrated in absolute ethanol and then embedded in paraffin wax. Six micron samples were stained routinely with Harris hematoxylin and erythrosine. Procedure of preparation-documentation was performed using the Quantament 500 Plus System. Stability of porcine pericardium after visible and ultraviolet light irradiation The aim of the present work was to evaluate the influence of the VIS- and UV-irradiation on the PP structure. Changes in the tissue structure stability were evaluated on the basis of SDS-PAGE electrophoresis and histological investigations as described in section . Stability of UV-irradiated tannic acid-crosslinked porcine pericardium The aim of present study was to evaluate the TA-modified PP stability after the tissue UV-irradiation. Changes in the stability of tissue structure were evaluated on the basis of SDS-PAGE electrophoresis and histological investigations as described in section . However two methods were used for staining gels the first with CBB and the second with silver. Investigated tissue samples were subjected to the SDS NaCl extraction and to enzymatic digestion in solution containing g of pancreatin P
