Báo cáo hóa học: "A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells | Nanoscale Res Lett 2009 4 903-912 DOI S11671-009-9333-7 NANO EXPRESS A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells Hsu-An Pan Yao-Ching Hung Chia-Wei Su Shih-Ming Tai Chiun-Hsun Chen Fu-Hsiang Ko G. Steve Huang Received 31 March 2009 Accepted 24 April 2009 Published online 19 May 2009 to the authors 2009 Abstract Micro-structures that mimic the extracellular substratum promote cell growth and differentiation while the cellular reaction to a nanostructure is poorly defined. To evaluate the cellular response to a nanoscaled surface NIH 3T3 cells were grown on nanodot arrays with dot diameters ranging from 10 to 200 nm. The nanodot arrays were fabricated by AAO processing on TaN-coated wafers. A thin layer of platinum 5 nm in thickness was sputtered onto the structure to improve biocompatibility. The cells grew normally on the 10-nm array and on flat surfaces. However 50-nm 100-nm and 200-nm nanodot arrays induced apoptosis-like events. Abnormality was triggered after as few as 24 h of incubation on a 200-nm dot array. For cells grown on the 50-nm array the abnormality started after 72 h of incubation. The number of filopodia extended from the cell bodies was lower for the abnormal cells. Immunostaining using antibodies against vinculin and actin filament was performed. Both the number of focal adhesions and the amount of cytoskeleton were decreased in cells grown on the 100-nm and 200-nm arrays. Precoatings of fibronectin FN or type I collagen promoted cellular anchorage and prevented the nanotopography- . Pan . Su . Tai . Ko G. Steve Huang El Institute of Nanotechnology National Chiao Tung University 1001 University Road Hsinchu 300 Taiwan ROC e-mail gstevehuang@ . Hung Section of Gynecologic Oncology Department of Obstetrics and Gynecology China Medical University and Hospital 91 Hsueh Shih Rd Taichung 404 Taiwan ROC . Chen Department of Mechanical Engineering .

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