Báo cáo hóa học: "Preparation and Characterization of Cationic PLA-PEG Nanoparticles for Delivery of Plasmid DNA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Research Preparation and Characterization of Cationic PLA-PEG Nanoparticles for Delivery of Plasmid DNA | Nanoscale Res Lett 2009 4 982-992 DOI s11671-009-9345-3 NANO EXPRESS Preparation and Characterization of Cationic PLA-PEG Nanoparticles for Delivery of Plasmid DNA Weiwei Zou Chunxi Liu Zhijin Chen Na Zhang Received 4 March 2009 Accepted 6 May 2009 Published online 21 May 2009 to the authors 2009 Abstract The purpose of the present work was to formulate and evaluate cationic poly lactic acid -poly ethyl-ene glycol PLA-PEG nanoparticles as novel non-viral gene delivery nano-device. Cationic PLA-PEG nanoparticles were prepared by nanoprecipitation method. The gene loaded nanoparticles were obtained by incubating the report gene pEGFP with cationic PLA-PEG nanoparticles. The physicochemical properties . morphology particle size surface charge DNA binding efficiency and biological properties . integrity of the released DNA protection from nuclease degradation plasma stability in vitro cytotoxicity and in vitro transfection ability in Hela cells of the gene loaded PLA-PEG nanoparticles were evaluated respectively. The obtained cationic PLA-PEG nanoparticles and gene loaded nanoparticles were both spherical in shape with average particle size of and nm polydispersity index of and zeta potentials of and mV respectively. The obtained cationic PLA-PEG nanoparticles with high binding efficiency 95 could protect the loaded DNA from the degradation by nuclease and plasma. The nanoparticles displayed sustained-release properties in vitro and the released DNA maintained its structural and functional integrity. It also showed lower cytotoxicity than Lipofectamine 2000 and could successfully transfect gene into Hela cells even in presence of serum. It could be concluded that the established gene loaded cationic PLA-PEG nanoparticles with excellent properties were promising non-viral nano-device which had potential to make cancer gene therapy achievable. W. Zou C. Liu Z. Chen N. Zhang El School of Pharmaceutical Science Shandong .

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