Tham khảo tài liệu 'insecticides pest engineering part 9', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Essential Plant Oils and Insecticidal Activity in Culex quinquefasciatus 229 The percentage of hatching inhibition was of the total number of larvae. The larvae completing their development to adults corresponded to of surviving larvae. As a figure of interest the greatest number of semi emergency in adults occurred at 72 hours after exposure to the oil of turpentine with a predominance of males . At 96 hour the largest proportion was in favor of females who became non-existent for this sex at 120 and 168 hours 5-7 d More recently oil of turpentine and the bicyclic a-pinene obtained by fractional distillation of oil of turpentine have been evaluated in Culex quinquefasciatus important vector of West Nile virus and encephalitis The purification of the a-pinene was made using column chromatography in system of variable stationary phase SiO2 Al2O3 and their mixtures in dependence of the polarities of the mobile phases for elution and separation . Molecular characterization was developed using techniques of FTIR and NMR as well as methodologies for its extension to field conditions type TLC chromatography on thin layer in the presence of plates of glass with silica gel 200-G254 activated with AgNO3 acetonitril via wet impregnation with methanol . The plates are eluted with mixtures of solvents of media polarity ethyl-acetone -alcohol isopropyl acetate . Figure 5 shows the spectroscopic characterization of the a pinene. In table 1 and 2 is shown the concentrations finally used the obtained mortalities and lethal concentrations that causes 50 to 95 percent of mortality the slope of the regression line and diagnostic dose. The lowest lethal concentrations were obtained with oils of turpentine and turpentine modified followed by Curcuma longa Psidium rotundatum and Chenopodium ambrosoide. By applying a x 2-square test p to the results of the bioassays the results showed that the mortalities are associated with the used dose. The higher slopes of the lines
