Tham khảo tài liệu 'insecticides pest engineering part 19', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Genetic Toxicological Profile of Carbofuran and Pirimicarb Carbamic Insecticides 529 Buenos Aires Argentina on mammalian Chinese hamster ovary cells as well as circulating erythrocytes of the fish Cnesterodon decemmaculatus and the amphibian Rhinella arenarum tadpoles were also estimated. A summary of the results obtained is presented in Fig. 3. The figure clearly reveals that all compounds assayed were able to inflict damage at chromosomal and cellular level regardless of the cellular system used as target. We observed that carbofuran Furadan and pirimicarb Aficida caused SCEs on mammalian cells indicating that they have a clastogenic activity Fig. 3A . It has been suggested that at the chromosomal level the induction of SCEs is a reliable indicator for the screening of clastogens since the bioassay is more sensitive than the analysis of clastogen-induced chromosomal aberrations Palitti et al. 1982 . The results also demonstrate the ability of pirimicarb Aficida to induce DNA damage quali- and quantitative analyzed by the frequency of chromosomal aberrations Fig. 3B . Furthermore a putative clastogenic aneugenic activity exerted in vitro by carbofuran Furadan and in vivo by pirimicarb Aficida was also demonstrated by the ability of the pesticide-induced micronuclei Fig. 3C . The analysis of the proliferative replication Fig. 3D and the mitotic indexes Fig. 3E demonstrated that both carbofuran Furadan and pirimicarb Aficida were able to delay the cell-cycle progression as well as to exert a marked reduction of the cellular mitotic activity on mammalian cells in vitro. Besides carbofuran Furadan and pirimicarb Aficida were able to induced a clear cellular cytotoxicity. This deleterious effect was estimated by a loss of lysosomal activity indicated by a decrease in the uptake of neutral red as well as alteration in energy metabolism measured by mitochondrial succinic dehydrogenase activity in the MTT assay as clearly revealed in insecticides-treated Chinese hamster .
