Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: One-Pot Green Synthesis and Bioapplication of L-ArginineCapped Superparamagnetic Fe3O4 Nanoparticles | Nanoscale Res Lett 2010 5 296-301 DOI S11671-009-9479-3 NANO EXPRESS Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow Sang-Wook Lee Haruyuki Kinoshita Hiroyuki Noji Teruo Fujii Takatoki Yamamoto Received 28 August 2009 Accepted 28 October 2009 Published online 14 November 2009 to the authors 2009 Abstract We have proposed and experimentally demonstrated that the measurement of the near-surface flow at the interface between a liquid and solid using a 10 nm-sized biomolecular motor of F1-ATPase as a nano-flowsensor. For this purpose we developed a microfluidic testbed chip to precisely control the liquid flow acting on the F1-ATPase. In order to visualize the rotation of F1-ATPase several hundreds nanometer-sized particle was immobilized at the rotational axis of F1-ATPase to enhance the rotation to be detected by optical microscopy. The rotational motion of F1-ATPase which was immobilized on an inner surface of the test-bed chip was measured to obtain the correlation between the near-surface flow and the rotation speed of F1-ATPase. As a result we obtained the relationship that the rotation speed of F1-ATPase was linearly decelerated with increasing flow velocity. The mechanism of the correlation between the rotation speed and the near-surface flow remains unclear however the concept to use biomolecule as a nano-flow-sensor was proofed successfully. Electronic supplementary material The online version of this article doi s11671-009-9479-3 contains supplementary material which is available to authorized users. S. -W. Lee H. Kinoshita T. Fujii Institute of Industrial Science University of Tokyo Fw-601 4-6-1 Komaba Meguro-ku Tokyo 153-8505 Japan H. Noji The Institute of Science and Industrial Research Osaka University 8-1 Mihogaoka-Ibaraki Osaka 567-0047 Japan T. Yamamoto El Department of Mechanical and Control Engineering Tokyo Institute of Technology Ishikawadai 1-314 Ookayama 2-12-1 Meguro-ku Tokyo 152-8550 Japan e-mail .
