Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Multi-Layered Films Containing a Biomimetic Stimuli-Responsive Recombinant Protein | Nanoscale Res Lett 2009 4 1247-1253 DOI s11671-009-9388-5 NANO PERSPECTIVES Multi-Layered Films Containing a Biomimetic Stimuli-Responsive Recombinant Protein J. S. Barbosa R. R. Costa A. M. Testera M. Alonso J. C. Rodriguez-Cabello J. F. Mano Received 1 April 2009 Accepted 2 July 2009 Published online 16 July 2009 to the authors 2009 Abstract Electrostatic self-assembly was used to fabricate new smart multi-layer coatings using a recombinant elastin-like polymer ELP and chitosan as the counterion macromolecule. The ELP was bioproduced purified and its purity and expected molecular weight were assessed. Aggregate size measurements obtained by light scattering of dissolved ELP were performed as a function of temperature and pH to assess the smart properties of the polymer. The build-up of multi-layered films containing ELP and chitosan using a layer-by-layer methodology was followed by quartz-crystal microbalance with dissipation monitoring. Atomic force microscopy analysis permitted to demonstrate that the topography of the multi-layered films could respond to temperature. This work opens new possibilities for the use of ELPs in the fabrication of biodegradable smart coatings and films offering new platforms in biotechnology and in the biomedical area. J. S. Barbosa R. R. Costa J. F. Mano El 3B s Research Group-Biomaterials Biodegradables and Biomimetics AvePark Zona Industrial da Gandra S. Claudio do Barco 4806-909 Caldas das Taipas Guimaraes Portugal e-mail jmano@ J. S. Barbosa R. R. Costa J. F. Mano IBB Institute for Biotechnology and Bioengineering PT Government Associated Laboratory Guimaraes Portugal A. M. Testera M. Alonso J. C. Rodriguez-Cabello . Bioforge Univ. Valladolid Edificio I D Paseo de Belen 1 47011 Valladolid Spain A. M. Testera M. Alonso J. C. Rodriguez-Cabello Networking Research Center on Bioengineering Biomaterials and Nanomedicine CIBER-BBN Valladolid Spain Keywords Elastin-like polymer Biodegradable polymers .
