Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction | Nanoscale Res Lett 2009 4 1409-1420 DOI s11671-009-9413-8 NANO EXPRESS The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction Cory Hanley Aaron Thurber Charles Hanna Alex Punnoose Jianhui Zhang Denise G. Wingett Received 3 June 2009 Accepted 7 August 2009 Published online 16 September 2009 to the authors 2009 Abstract Nanotechnology represents a new and enabling platform that promises to provide a range of innovative technologies for biological applications. ZnO nanoparticles of controlled size were synthesized and their cytotoxicity toward different human immune cells evaluated. A differential cytotoxic response between human immune cell subsets was observed with lymphocytes being the most resistant and monocytes being the most susceptible to ZnO nanoparticle-induced toxicity. Significant differences were also observed between previously activated memory lymphocytes and naive lymphocytes indicating a relationship between cell-cycle potential and nanoparticle susceptibility. Mechanisms of toxicity involve the generation of reactive oxygen species with monocytes displaying the highest levels and the degree of cytotoxicity dependent on the extent of nanoparticle interactions with cellular membranes. An inverse relationship between nanoparticle size and cytotoxicity as well as nanoparticle size and reactive oxygen species production was observed. In addition ZnO nanoparticles induce the production of the proinflammatory C. Hanley D. G. Wingett H Department of Biological Sciences Boise State University Boise ID 83725 USA e-mail denisewingett@ A. Thurber C. Hanna A. Punnoose J. Zhang Department of Physics Boise State University 1910 University Dr. Boise ID 83725 USA D. G. Wingett Department of Medicine Division of Gerontology and Geriatric Medicine University of Washington Seattle WA 98195 USA D. G. Wingett MSTMRI Research Institute of St. Luke s Regional Medical Center Boise ID 83712 USA cytokines
