Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Fatty Acid Binding Domain Mediated Conjugation of Ultrafine Magnetic Nanoparticles with Albumin Protein | Nanoscale Res Lett 2009 4 138-143 DOI S11671-008-9213-6 NANO EXPRESS Fatty Acid Binding Domain Mediated Conjugation of Ultrafine Magnetic Nanoparticles with Albumin Protein D. K. Bora P. Deb Received 4 September 2008 Accepted 11 November 2008 Published online 22 November 2008 to the authors 2008 Abstract A novel bioconjugate of stearic acid capped maghemite nanoparticle c-Fe2O3 with bovine serum albumin BSA was developed by taking recourse to the fatty acid binding property of the protein. From FT-IR study it was found that conjugation took place covalently between the amine group of protein molecule and carboxyl group of stearic acid capped maghemite nanoparticle. TEM study further signified the morphology of the proposed nanobioconjuagte. The binding constant of nanoparticle with protein molecule was evaluated from the optical property studies. Also magnetic measurement M-H showed retaining of magnetic property by significant values of saturation magnetization and other hysteretic parameters. Keywords Bioconjugate Maghemite nanoparticle Bovine serum albumin Covalent interaction Fatty acid binding domain Introduction Bioconjugation of magnetic nanoparticles is basically done to make it compatible for numerous biomedical applications such as MRI contrast enhancement drug delivery detoxification of biological fluids immunoassay tissue D. K. Bora P. Deb H Department of Physics Tezpur University Central University Napaam Tezpur 784028 India e-mail pdeb@ Present Address D. K. Bora Department of Biotechnology Indian Institute of Technology Guwahati North Guwahati 781039 India repair hyperthermia etc. 1-6 . Besides these bioconjugate systems are also being applied in various large-scale bioprocesses such as nucleic acid detachment protein separation magnetic biosensor etc. 7-9 . All these biorelated applications require the use of magnetic nanoparticles that should have size smaller than 10 nm with overall narrow particle size distribution so that .
