Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of pH on the Interaction of Gold Nanoparticles with DNA and Application in the Detection of Human p53 Gene Mutation | Nanoscale Res Lett 2009 4 216-220 DOI s11671-008-9228-z NANO EXPRESS Effect of pH on the Interaction of Gold Nanoparticles with DNA and Application in the Detection of Human p53 Gene Mutation Liping Sun Zhaowu Zhang Shuang Wang Jianfeng Zhang Hui Li Lei Ren Jian Weng Qiqing Zhang Received 22 October 2008 Accepted 25 November 2008 Published online 10 December 2008 to the authors 2008 Abstract Gold nanoparticles GNPs are widely used to detect DNA. We studied the effect of pH on the assembly disassembly of single-stranded DNA functionalized GNPs. Based on the different binding affinities of DNA to GNPs we present a simple and fast way that uses HCl to drive the assembly of GNPs for detection of DNA sequences with single nucleotide differences. The assembly is reversible and can be switched by changing the solution pH. No covalent modification of DNA or GNP surface is needed. Oligonucleotide derived from human p53 gene with one-base substitution can be distinguished by a color change of the GNPs solution or a significant difference of the maximum absorption wavelength kmax compared with wildtype sequences. This method enables detection of 10 picomole quantities of target DNA. Keywords Single-stranded DNA HCl Gold nanoparticles p53 Mutation L. Sun Z. Zhang S. Wang J. Zhang H. Li L. Ren J. Weng Q. Zhang Department of Biomaterials College of Materials Xiamen University Xiamen 361005 China e-mail sunliping@ Q. Zhang e-mail zhangqiq@ L. Ren State Key Laboratory for Physical Chemistry of Solid Surfaces Xiamen University Xiamen 361005 China Q. Zhang Institute of Biomedical Engineering Chinese Academy of Medical Science and Peking Union Medical College Tianjin 300192 China Introduction Gold nanoparticles GNPs coupled with biomolecules are of great current interest because of their biomedical applications. GNPs have been used to detect DNA with high sensitivity and selectivity 1 . Mutations single nucleotide polymorphisms SNPs chromosomal .
