Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals | Nanoscale Res Lett 2009 4 229-233 DOI s11671-008-9230-5 NANO EXPRESS Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals Daisuke Asai Jeong-Hun Kang Riki Toita Akira Tsuchiya Takuro Niidome Hideki Nakashima Yoshiki Katayama Received 11 September 2008 Accepted 2 December 2008 Published online 17 December 2008 to the authors 2008 Abstract Recently we have proposed a novel strategy for a cell-specific gene therapy system based on responses to intracellular signals. In this system an intracellular signal that is specifically and abnormally activated in the diseased cells is used for the activation of transgene expression. In this study we used protein kinase C PKC a as a trigger to activate transgene expression. We prepared a PKCa-responsive polymer conjugate PPC S and a negative control conjugate PPC A in which the phosphorylation site serine Ser was replaced with alanine Ala . The phosphorylation for polymer DNA complexes was determined with a radiolabel assay using y-32P ATP. PPC S DNA complexes were phosphorylated by the addition of PKCa but no phosphorylation of the PPC A Daisuke Asai and Jeong-Hun Kang contributed equally to this work. D. Asai H. Nakashima Department of Microbiology St. Marianna University School of Medicine 2-16-1 Sugao Miyamae-ku Kawasaki 216-8511 Japan D. Asai . Kang H. Nakashima Y. Katayama CREST Japan Science and Technology Corporation 4-1-8 Honcho Kawaguchi-shi Saitama 332-0012 Japan . Kang T. Niidome Y. Katayama El Department of Applied Chemistry Faculty of Engineering Kyushu University 744 Motooka Nishi-Ku Fukuoka 819-0395 Japan e-mail ykatatcm@ R. Toita A. Tsuchiya T. Niidome Y. Katayama Graduate School of Systems Life Sciences Kyushu University Fukuoka 819-0395 Japan T. Niidome Y. Katayama Center for Future Chemistry Kyushu University 744 Motooka Nishi-Ku Fukuoka 819-0395 Japan DNA complex was observed. Moreover after microinjection of polymer .
