Tham khảo tài liệu 'advances in applied biotechnology part 2', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Biotechnology of Agricultural Wastes Recycling Through Controlled Cultivation of Mushrooms 9 experiments were conducted under the following conditions temperature 25 C agitation speed 120-180 rev min-1 initial pH . After 10-12 d of incubation the fungal cultures were ready to be inoculated aseptically into the glass vessel of 20 l laboratory-scale bioreactor that was designed to be used for controlled submerged cultivation of edible and medicinal mushrooms on substrata made of wastes resulted from the industrial processing of cereal grains Fig. 5 . Fig. 5. General view of the Laboratory scale bioreactor 15 L After a period of submerged fermentation lasting up to 120 h small mushroom pellets developed inside the nutritive broth Fig. 6 7 . Fig. 6. Mycelial biomass of G. lucidum collected after submerged fermentation 10 Advances in Applied Biotechnology Fig. 7. Mycelial biomass in the shape of fungal pellets of L. edodes collected after submerged fermentation The fermentation process was carried out by inoculating the growing medium volume 10 000 ml with mycelia inside the culture vessel of the laboratory-scale bioreactor. The whole process of growing lasts for a single cycle between 5-7 days in case of L. edodes and between 3 to 5 days for G. lucidum. The strains of these fungal species were characterized by morphological and cultural stability proven by their ability to maintain the phenotypic and taxonomic identities. The experiments were carried out in three repetitions. Observations on morphological and physiological characters of these two tested species of fungi were made after each culture cycle highlighting the following aspects sphere-shaped structure of fungal pellets sometimes elongated irregular with various sizes from 2 to 5 mm in diameter reddish-brown colour - G. lucidum culture Fig. 8 . Fig. 8. Stereomicroscopic view of G. lucidum pellets after controlled submerged fermentation Biotechnology of Agricultural Wastes Recycling Through Controlled .
