Tham khảo tài liệu 'cell metabolism cell homeostasis and stress response part 12', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 156 Cell Metabolism - Cell Homeostasis and Stress Response glass substrate were then dehydrated in a series of increasing ethanol concentrations 30 50 70 95 and 100 and immersed in 1 1 1 3 3 3-hexamethyldisilazane HMDS Acros Organics Springfield NJ USA for 90 minutes and stored in a desiccator for 24 hours. The cover glasses were then mounted on metal stubs sputter-coated with gold and Hela cells morphology was examined by SEM JEOL-JMS-T33A Scanning Microscope Tokyo Japan . Each experimental treatment was repeated five times. The results obtained presented heterocedasticity. The Kruskall-Wallis and a post-hoc Dunn tests were used to detect differences in absorbance values MTT among investigated groups. Differences were considered statistically significant at p . Results The box plot Fig. 1 shows the median 1st and 3rd quartiles lowest and highest values obtained under each of the experimental and control conditions. Considering the negative control group C-L- as having 100 of Hela cell viability a significant reduction in cell metabolism of and was observed for curcumin concentrations of 5 10 and 20 ụM when the cells were irradiated by the blue LED. Fig. 1. Summary of absorbance values obtained after experiments with Hela cell culture. In the C L groups there was statistically significant difference p between the CUR concentrations of 5 and 20 ụM Table 1 . In comparison with the control group C-L- the dark toxicity of CUR was observed for the concentrations of 10 and 20 ụM reduction of and respectively although no significant differences were detected among the three concentrations. The irradiation control group showed similar metabolism activity to the negative control group showing that the light dose used in the present study did not alter the cell metabolism. Photodynamic Therapy to Eradicate Tumor Cells 157 Experimental and control conditions Mean Ranks Post hoc test C-L- a C L- 5pM abc C L- 10pM c C L-
