Tham khảo tài liệu 'fungicides for plant and animal diseases part 8', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | In Vitro Multiplication of Aromatic and Medicinal Plants and Fungicide Activity 131 were dispensed into sterilized Petri dishes 9 cm . After solidification a mycelial disk of 4 mm diameter of the test Aspergillus fumigatus taken from 4 days -old fungi culture was placed at the center of the medium. The mycelial disks on PDA without any test constituents were performed in the same way and used as control. Radial growth of colonies was measured at two points along the diameter of the plate and the mean of these two readings was taken as the diameter of the fungal colony. After incubation at 25 C in darkness growth zones were measured at the third fifth and the seventh day. The growth of the colonies in control sets was compared with that of various treatments and the difference was converted into percent inhibition c -T x 100 c where c and T are the radial diameters of the colony in control and treatment respectively. The percentage of growth inhibition is expressed as a mean of three replicate tests for each treatment. The complete antifungal analysis was carried out under strict aseptic conditions Zhang et al. 2006 . The analyses were performed using SPSS Statistical Package for the Social Sciences version . The one-way analysis of variance ANOVA followed by Tukey s Test with P were used to detect significant differences in inhibition fungi. Results Effect of four different concentrations 5 mg mL 10 mg mL 20 mg mL and 25 mg mL of Thymus and Mentha extract plants was tested against Aspergillus fumigatus. Antifungal activity was assayed and data on effect of plant extracts on the growth of Aspergillus fumigatus in the third fifth and seventh day is presented in Table 4. The data revealed that reduction in growth of Aspergillus fumigatus was observed with extracts of Thymus and Mentha. Plant species Inhibition of Aspergillus fumigatus Third day Fifth day Seventh day Concentrations of aqueous plant extracts in PDA mg mL 5 10 20 25 5 10 20 25 5
