Tham khảo tài liệu 'insecticides basic and other applications part 6', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Development of a Prophylactic Butyrylcholinesterase Bioscavenger to Protect Against InSecticide Toxicity Using a Homologous Macaque Model 89 terminating in sialic acid Paccalet et al. 2007 Castilho et al. 2010 . In addition different glycoforms of plant derived proteins can be generated by protein targeting to different compartments i cytosol aglycosylated ii ER high mannose or iii secreted into the apoplast complex Stoger et al. 2005 Effects of the route of administration on pharmacokinetics As mentioned delivery of PEG-rBChE as a pre-exposure modality is disadvantaged by its large size and a 1 1 stoichiometry between the enzyme and OP requiring high doses due to the high LD50 of many insecticides ug-mg kg levels . The route of systemic delivery of high doses of native BChE MW 350KDa and tetrameric PEG-rMaBChE MW 800KDa will determine the pharmacokinetics PK of clearance and is critical to efficacy and safety. Currently very little monkey data exists on the delivery of a stoichiometrically equivalent dose of PEG-rBChE calculated to protect against a known LD50 of a toxic OP insecticide. Although immediate release requiring intravenous iv injection may be necessary in certain high threat situations these are usually impractical in the field. Needleless cutaneous delivery via the dermis and epidermis chemical mediators electroporation appear quite promising but are unlikely to deliver high doses. Thus self-administered transdermal injections through the skin either by subcutaneous sc or intramuscular im routes have been the approaches most commonly used virtually all human vaccines currently on the market being administered via these routes. Traditionally autoinjectors devices for im delivery of a self administered single dose of a drug are used in the military to protect personnel from chemical warfare agents and are currently used to deliver morphine for pain and atropine diazepam and 2-PAM-Cl for first-aid against nerve agents. For this reason most animal .
